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US 2006/0216756 A1Published application
Method for evaluating oxidized protein in horny cell layer
AssigneeShiseido Company, Ltd.
InventorsHiroshi Fujita, Tetsuji Hirao
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AssigneeShiseido Company, Ltd.
InventorsHiroshi Fujita, Tetsuji Hirao
Estimating the term…
The invention provides a method for two-dimensional evaluation of the nature of horny layer oxidized protein on a horny layer, the method comprising specific fluorescent labeling of the carbonyl groups of the oxidized protein in a horny layer specimen taken from skin, and detection of the fluorescence for evaluation, as well as a kit used to carry out the method and a method for screening of agents which inhibit increase in oxidized protein. The invention further provides a method for detecting the oxidized form of a cornified envelope consisting of the water-insoluble substances in a skin-derived horny layer specimen, the method comprising specific fluorescent labeling of the carbonyl groups in a cornified envelope in the oxidized form and detection of the fluorescence thereof for evaluation.
A method for two-dimensional evaluation of the nature of horny layer oxidized protein on a horny layer, the method being characterized by comprising the steps of specific fluorescent labeling of the carbonyl groups of said oxidized protein in a horny layer specimen taken from skin, and detection of the fluorescence for evaluation.
A kit to be used in a method for two-dimensional evaluation of the presence of horny layer oxidized protein on a horny layer, the kit being characterized by comprising:
A screening method for agents which inhibit oxidized protein increase, the method being characterized by comprising the steps of treating a horny layer with an appropriate oxidizing agent and candidate agent, and then accomplishing specific fluorescent labeling of the carbonyl groups of the oxidized protein on the horny layer and detecting the fluorescence to evaluate the activity of said agent for inhibiting oxidized protein increase.
A method for detecting the oxidized form of a cornified envelope consisting of the water-insoluble substances in a skin-derived horny layer specimen, the method being characterized by specific fluorescent labeling of the carbonyl groups in a cornified envelope in said oxidized form and detection of the fluorescence thereof for evaluation.
Dependencies are inferred from the claim text.