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US 2006/0216746 A1Published application
Novel protein and DNA thereof
AssigneeTakeda Chemical Industries, Ltd.
InventorsKoji Yoshimura, Yuichi Hikichi, Atsushi Nishimura
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AssigneeTakeda Chemical Industries, Ltd.
InventorsKoji Yoshimura, Yuichi Hikichi, Atsushi Nishimura
Estimating the term…
The present invention relates to a novel protein belonging to an ADAM family, a partial peptide thereof or a salt thereof, a DNA encoding the protein, a recombinant vector comprising the DNA, a transformant, a method for producing the protein, a medicine comprising the protein or the DNA, an antibody against the protein, a method/kit for screening for a compound or a salt thereof which promotes or inhibits the protease activity or the extracellular metric degrading enzyme activity (preferably, the peptidoglycan degrading enzyme activity) of the protein, a compound obtained by the screening, and a medicine comprising the compound. The present protein and a DNA encoding it can be used, for example, as an agent for treating or preventing various diseases such as disc herniation, ischialgia, glomerular nephritis, diabetic nephropathy, hepatic fibrosis, pulmonary fibrosis or osteopetrosis. In addition, the present antibody can be used for quantitating the present protein in a test solution. Further, the present protein is useful as a reagent for screening for a compound which promotes or inhibits the protease activity of the present protein.
An isolated protein or a salt thereof which has an amino acid sequence represented by SEQ ID No:
A compound or a salt thereof which promotes or inhibits the protease activity and is obtainable by using the method for screening according to claim 19.
A medicine which comprises a compound or a salt thereof which promotes or inhibits the protease activity and is obtainable by the screening method according to claim 19.
A method for detecting a proteoglycan degrading enzyme gene, which comprises mixing and culturing a transformant in which a test gene is introduced and an animal-derived cell producing cartilage or cartilage matrix, and measuring glycosaminoglyean sulfate in the culture supernatant.
A method for screening an agent for inhibiting or promoting the proteoglycan degrading enzyme activity, which comprises mixing and culturing (i) a recombinant in which a gene encoding a protein having the proteoglycan degrading enzyme activity is introduced, (ii) an animal-derived cell producing cartilage or cartilage matrix, and (iii) a test compound, and measuring glycosaminoglycan sulfate in the culture supernatant.
A non-human mammal which has a DNA having a DNA having a base sequence encoding an protein having an amino acid sequence identical or substantially identical to an amino acid sequence represented by SEQ ID No:
Dependencies are inferred from the claim text.