Human aminoacyl-tRNA synthetase polypeptides useful for the regulation of angiogenesis
AssigneeThe Scripps Research Institute
InventorsPaul Schimmel, Keisuke Wakasugi
Life of this patent
Estimating the term…
Abstract
Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.
Claims41
An isolated nucleic acid comprising a polynucleotide encoding a mature polypeptide and having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a polynucleotide of SEQ ID NO:
- 9;
- (b) a polynucleotide which is hybridizable to a polynucleotide of SEQ ID NO:
- 9;
- (c) a polynucleotide encoding a polypeptide comprising a Rossmann fold nucleotide binding domain, wherein the isolated polypeptide is capable of regulating vascular endothelial cell function;
- (d) a polynucleotide that is hybridizable to a polynucleotide encoding a polypeptide comprising a Rossmann fold nucleotide binding domain, which is capable of regulating vascular endothelial cell function;
- (e) a polynucleotide encoding a polypeptide selected from the group consisting of:
- a polypeptide consisting essentially of amino acid residues 48-471 of SEQ ID NO:10;
- a polypeptide consisting essentially of amino acid residues 71-471 of SEQ ID NO:10;
- a polypeptide of approximately 47 kD molecular weight produced by cleavage of the polypeptide of SEQ ID NO:10 with polymorphonuclear leucocyte elastase;
- and
- angiostatic fragments thereof comprising the amino acid sequence -Asp-Leu-Thr-;
- (f) a polynucleotide that is hybridizable to a polynucleotide encoding a polypeptide selected from the group consisting of:
- a polypeptide consisting essentially of amino acid residues 48-471 of SEQ ID NO:10;
- a polypeptide consisting essentially of amino acid residues 71-471 of SEQ ID NO:10;
- a polypeptide of approximately 47 kD molecular weight produced by cleavage of the polypeptide of SEQ ID NO:10 with polymorphonuclear leucocyte elastase;
- and
- angiostatic fragments thereof comprising the amino acid sequence -Asp-Leu-Thr-;
- (g) a polynucleotide encoding an epitope of a polypeptide of SEQ ID NO:10;
- and
- (h) a polynucleotide that is hybridizable to a polynucleotide encoding an epitope of a polypeptide of SEQ ID NO:10.
An isolated, truncated tryptophanyl-tRNA synthetase polypeptide comprising a Rossmann fold nucleotide binding domain, wherein the polypeptide is capable of regulating vascular endothelial cell function, wherein the polypeptide is produced by treating tryptophanyl-tRNA synthetase with a protease.
An isolated, truncated tryptophanyl-tRNA synthetase polypeptide comprising a Rossmann fold nucleotide binding domain, wherein the polypeptide is capable of regulating vascular endothelial cell function, the polypeptide being selected from the group consisting of;
- (a) a polypeptide consisting essentially of amino acid residues 48-471 of SEQ ID NO:10;
- (b) a polypeptide consisting essentially of amino acid residues 71-471 of SEQ ID NO:10;
- (c) a polypeptide of approximately 47 kD molecular weight produced by cleavage of the polypeptide of SEQ ID NO:10 with polymorphonuclear leucocyte elastase;
- and
- (d) angiostatic fragments thereof comprising the amino acid sequence -Asp-Leu-Thr-;
- wherein the polypeptide optionally includes a secretory peptide sequence fused to the N-terminus thereof.
Dependencies are inferred from the claim text.