Sequencing method
AssigneeGe Healthcare UK Limited
InventorRaj Odedra
Life of this patent
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Abstract
Disclosed is a single molecule sequencing method comprising the steps of: a) attaching a molecule to a solid phase; b) incubating the molecule with a first composition comprising a first reporter moiety; c) detecting incorporation of said first reporter moiety; d) performing a reaction to eliminate said first reporter moiety; e) incubating the molecule with a second composition comprising a second reporter moiety; and f) detecting incorporation of said second reporter moiety; characterized in that the first and second reporter moieties can be distinguished from each other.
Claims15
A single molecule sequencing method comprising the steps of
- a) attaching a molecule to a solid phase
- b) incubating the molecule with a first composition comprising a first reporter moiety
- c) detecting incorporation of said first reporter moiety
- d) performing a reaction to eliminate said first reporter moiety
- e) incubating the molecule with a second composition comprising a second reporter moiety
- f) detecting incorporation of said second reporter moiety characterised in that the first and second reporter moieties can be distinguished from each other.
A method for nucleic acid sequencing comprising the steps of
- a) attaching a primer/template complex to a solid phase
- b) incubating the primer/template complex in the presence of a first composition comprising at least one reporter moiety and a polymerase
- c) detecting incorporation of said first reporter moiety
- d) performing a reaction to eliminate said first reporter moiety
- e) incubating the primer/template complex in the presence of a second composition comprising a second reporter moiety and a polymerase
- f) detecting incorporation of said second reporter moiety characterised in that the first and second reporter moieties can be distinguished from each other.
A method of detecting errors in a sequencing reaction comprising the steps of
- a) attaching a molecule to a solid phase
- b) incubating the molecule with a first composition comprising a first reporter moiety
- c) detecting incorporation of said first reporter moiety
- d) performing a reaction to eliminate said first reporter moiety
- e) incubating the molecule with a second composition comprising a second reporter moiety
- f) detecting incorporation of said second reporter moiety and detecting presence of said first reporter moiety as an indication of a false base call characterised in that the first and second reporter moieties can be distinguished from each other and wherein the presence of the first reporter moiety after the elimination step d) is indicative of an error.
Dependencies are inferred from the claim text.