Labeling reagent
AssigneeRoche Diagnostics Opertions, Inc.
InventorsDieter Heindl, Thomas Froehlich, Heribert Maerz, Monika Seller
Life of this patent
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Abstract
The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.
Claims20
A compound having the formula
- wherein
- A is selected from the group consisting of H, phosphate, triphosphate, a protecting group, and a chain of nucleotide residues,
- B is selected from the group consisting of H, a phosphoramidite group, an H-phosphonate, a solid support, and a chain of nucleotide residues,
- R is selected from the group consisting of H, F, OH, O-alkyl, O-alkenyl, O-alkinyl, and an O-protective group
- one of W1 and W2 is C—NO2, and the other of W1 and W2 is CH or N,
- one of X1, X2, X3, and X4 is selected from the group consisting of a reactive group, a protected reactive group, a linker with a reactive group, a linker with a protected reactive group, a signal entity, a protected signal entity, a linker with a signal entity, and a linker with a protected signal entity, and the remaining three of X1, X2, X3, and X4 are each H.
Dependencies are inferred from the claim text.