Glomerular expression profiling
OwnerChrister Betsholtz, Karl Tryggvason +3inventors
InventorsChrister Betsholtz, Karl Tryggvason, Minoru Takemoto, Liqun He, Jaakko Patrakkas
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions comprising various glomerular probe sets and methods for their use.
Claims24
A composition comprising a plurality of isolated probes that in total selectively bind to at least 2 the glomerular markers listed in Table 6 or Table 7, complements thereof, or their expression products, wherein at least 10% of the probes in total are selective for glomerular markers.
A composition comprising a plurality of isolated probes that in total selectively bind to at least 51 of the glomerular markers disclosed herein in Table 9, complements thereof, human homologues thereof, or their expression products, wherein at least 3.75% of the probes in total are selective for glomerular markers.
A method for identifying glomerular marker polynucleotides, comprising
- a) perfusing a target kidney in an organism with a solution containing magnetic beads, wherein the magnetic bead diameter is approximately equivalent to the capillary diameter of glomerular capillaries;
- b) removing glomerular-containing kidney tissue from the organism;
- c) digesting the glomerular-containing kidney tissue to separate glomeruli from associated kidney tissue;
- d) magnetically isolating glomeruli from the digested glomerular-containing kidney tissue;
- e) isolating mRNA from the isolated glomeruli
- f) normalizing the mRNA to at least partially suppress high copy number mRNA transcripts;
- g) identifying mRNA that are expressed in the glomerulus, wherein such mRNA are glomerular marker polynucleotides.
Dependencies are inferred from the claim text.