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US 2006/0216705 A1Published application
Transcription activation complex and utilization thereof
AssigneeSumitomo Chemical Company, Limited
InventorNorihisa Ohe
Life of this patent
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Abstract
The present invention provides a complex of the transcription coupling factor of any of ARNT 1 to 3 and the transcription regulatory factor comprising any of the amino acid sequence from the amino acid sequence group comprising for example the amino acid sequences represented by SEQ ID Nos.1 to 3, which is a transcription activating complex having an ability of binding to a DNA region (5′-ACGTG-3′, SEQ ID No.16) to which a transcription inhibiting complex of the transcription coupling factor and a Sim2 as a transcription regulatory factor can be bound and having an ability of promoting the transcription of a gene located downstream of the DNA region.
Claims20
a complex of the transcription coupling factor of any of ARNT 1 to 3 and the transcription regulatory factor comprising any of the amino acid sequences shown below, which is a transcription activating complex having an ability of binding to a DNA region (5′-ACGTG-31, SEQ ID No.16) to which a transcription inhibiting complex of a Sim2 as a transcription regulatory factor and the transcription coupling factor can be bound and having an ability of promoting the transcription of a gene located downstream of the DNA region, wherein the amino acid sequences are:
- <<Amino Acid Sequence Group>>
- (a) the amino acid sequence represented by any of SEQ ID Nos.1 to 3,
- (b) the amino acid sequence of a protein comprising an amino acid sequence exhibiting an amino acid identity of 90% or more to the amino acid sequence represented by any of SEQ ID Nos.1 to 3 and also having a transcription regulation ability,
- (c) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 102 to 2507 in the nucleotide sequence represented by SEQ ID No.4 and also having a transcription regulation ability,
- (d) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 51 to 2456 in the nucleotide sequence represented by SEQ ID No.5 and also having a transcription regulation ability, and
- (e) the amino acid sequence a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 35 to 2440 in the nucleotide sequence represented by SEQ ID No.6 and also having a transcription regulation ability.
3 more elements
a transformant obtainable by introducing a single vector comprising the both of the DNAs shown below or several vectors comprising such DNAs independently into a host cell, wherein the DNAs are:
- <<DNAs>>
- (1) the DNA comprising a nucleotide sequence encoding the amino acid sequence of the transcription coupling factor of any of ARNT 1 to 3, and
- (2) the DNA comprising a nucleotide sequence encoding any of the amino acid sequences shown below:
- <<Amino Acid Sequence Group>>
- (a) the amino acid sequence represented by any of SEQ ID Nos.1 to 3,
- (b) the amino acid sequence of a protein comprising an amino acid sequence exhibiting an amino acid identity of 90% or more to the amino acid sequence represented by any of SEQ ID Nos.1 to 3 and also having a transcription regulation ability,
- (c) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 102 to 2507 in the nucleotide sequence represented by SEQ ID No.4 and also having a transcription regulation ability,
- (d) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 51 to 2456 in the nucleotide sequence represented by SEQ ID No.5 and also having a transcription regulation ability, and
- (e) the amino acid sequence a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 35 to 2440 in the nucleotide sequence represented by SEQ ID No.6 and also having a transcription regulation ability.
6 more elements
a use as a Down's syndrome improving agent a single vector comprising the both of the DNAs shown below or several vectors comprising said DNAs independently, wherein the DNAs are:
- <<DNAs>>
- (1) the DNA comprising a nucleotide sequence encoding the amino acid sequence of the transcription coupling factor of any of ARNT 1 to 3;
- (2) the DNA comprising a nucleotide sequence encoding any of the amino acid sequences shown below:
- <<Amino Acid Sequence Group>>
- (a) the amino acid sequence represented by any of SEQ ID Nos.1 to 3,
- (b) the amino acid sequence of a protein comprising an amino acid sequence exhibiting an amino acid identity of 90% or more to the amino acid sequence represented by any of SEQ ID Nos.1 to 3 and also having a transcription regulation ability,
- (c) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 102 to 2507 in the nucleotide sequence represented by SEQ ID No.4 and also having a transcription regulation ability,
- (d) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 51 to 2456 in the nucleotide sequence represented by SEQ ID No.5 and also having a transcription regulation ability, and
- (e) the amino acid sequence a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 35 to 2440 in the nucleotide sequence represented by SEQ ID No.6 and also having a transcription regulation ability.
6 more elements
a transcription activating complex containing a protein comprising one member (A or B) among the member I shown below and one member (X or Y) among the member II shown below and a protein comprising the other member (B or A) among the member I shown below and the other member (Y or X) among the member II shown below, the formation of the complex with the both protein being under the control by the ligand, the member I being:
- <<Member I>>
- (A) a region to which a transcription regulatory factor comprising any of the following amino acid sequences is bound in the transcription coupling factor of any of ARNT 1 to 3;
- or,
- (B) a region to which the transcription coupling factor of any of ARNT 1 to 3 is bound in a transcription regulatory factor comprising any of the following amino acid sequences;
- the member II being:
- <<Member II>>
- (X) a DNA binding region of a transcription regulatory factor which is functional in a host cell;
- or,
- (Y) a transcription activating region of a transcription regulatory factor which is functional in a host cell;
- <<Amino Acid Sequence Group>>
- (a) the amino acid sequence represented by any of SEQ ID Nos.1 to 3,
- (b) the amino acid sequence of a protein comprising an amino acid sequence exhibiting an amino acid identity of 90% or more to the amino acid sequence represented by any of SEQ ID Nos.1 to 3 and also having a transcription regulation ability,
- (c) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 102 to 2507 in the nucleotide sequence represented by SEQ ID No.4 and also having a transcription regulation ability,
- (d) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 51 to 2456 in the nucleotide sequence represented by SEQ ID No.5 and also having a transcription regulation ability, and
- (e) the amino acid sequence a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 35 to 2440 in the nucleotide sequence represented by SEQ ID No.6 and also having a transcription regulation ability.
12 more elements
a transformant obtainable by introducing:
- (1) one member (a or b) among the member i shown below and one member (x or y) among the member ii shown below;
- (2) the other member (b or a) among the member i shown below and the other member (y or x) among the member ii shown below;
- and;
- (3) the member iii shown below,
- the member i being:
- <<Member i>>
- (a) the DNA comprising a nucleotide sequence encoding the amino acid sequence of the region to which a transcription regulatory factor comprising any of the amino acid sequences shown below is bound in the transcription coupling factor of any of ARNT 1 to 3;
- (b) the DNA comprising a nucleotide sequence encoding the amino acid sequence of the region to which the transcription coupling factor of any of ARNT 1 to 3 is bound in the transcription regulatory factor comprising any of the amino acid sequences shown below;
- the member ii being:
- <<Member ii>>
- (X) a DNA comprising a nucleotide sequence encoding the amino acid sequence of a DNA binding region of a transcription regulatory factor which is functional in a host cell;
- or,
- (Y) a DNA comprising a nucleotide sequence encoding the amino acid sequence of a transcription activating region of a transcription regulatory factor which is functional in a host cell;
- the member iii being:
- <<Member iii>>
- a DNA comprising a reporter gene connected to the downstream of the promoter, said promoter contains a DNA to which a DNA binding region comprising the amino acid sequence encoded by the nucleotide sequence of (x) among the member ii can be bound;
- <<Amino Acid Sequence Group>>
- (a) the amino acid sequence represented by any of SEQ ID Nos.1 to 3,
- (b) the amino acid sequence of a protein comprising an amino acid sequence exhibiting an amino acid identity of 90% or more to the amino acid sequence represented by any of SEQ ID Nos.1 to 3 and also having a transcription regulation ability,
- (c) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 102 to 2507 in the nucleotide sequence represented by SEQ ID No.4 and also having a transcription regulation ability,
- (d) the amino acid sequence of a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 51 to 2456 in the nucleotide sequence represented by SEQ ID No.5 and also having a transcription regulation ability, and
- (e) the amino acid sequence a protein comprising an amino acid sequence encoded by a DNA which hybridizes under a stringent condition with a DNA consisting of the nucleotide sequence represented by the nucleotide numbers 35 to 2440 in the nucleotide sequence represented by SEQ ID No.6 and also having a transcription regulation ability.
19 more elements
Dependencies are inferred from the claim text.