Water-soluble conjugates for electrochemical detection

Abstract

The present invention provides methods of detecting the presence or amount of an analyte in a sample. The methods involve contacting a surface coated with a specific binding molecule to the analyte to be detected with the sample, contacting the surface coated with the specific binding molecule to the analyte to be detected with a water-soluble conjugate having at least one carrier component, at least one linking component, at least one electrochemical signal enzyme covalently attached to the carrier component, at least one targeting element for the analyte to be detected, and forming a binding complex of the specific binding molecule coated on the surface, the analyte, and the water-soluble conjugate. The binding complex is contacted with a substrate for an electrochemical signal enzyme to form a product solution, and the presence or absence of analyte in the sample is determined.

Dependencies are inferred from the claim text.