Extraction and concentration method
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsHenry Stone, Cam Greene, Peter Holt, Richard Gast, Kunho Seo
Life of this patent
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Abstract
A method for extraction and concentration of antibodies, antigens, bacteria and virus from biological samples. The method also provides a preparation that is suitable for use as a vaccine. The method includes the addition of liquid carboxylic acids and a centrifugation step.
Claims13
1-4. (canceled)
A method for extraction of a biological sample consisting essentially of:
- (a) adding at least one liquid carboxylic acid to a biological sample to form a first composition,
- (b) mixing said first composition to form a second composition that contains an aqueous soluble product,
- (c) centrifuging said second composition to form an aqueous layer and and oily layer, and
- (d) harvesting said aqueous layer to produce a concentrated sample selected from the group consisting of proteins, bacteria, virus, and mixtures thereof.
6-12. (canceled)
A method for obtaining purified antibodies from a biological sample consisting essentially of:
- (a) adding at least one liquid carboxylic acid to a sample to form a first composition,
- (b) mixing said first composition to form a second composition that contains an aqueous soluble product,
- (c) centrifuging said second composition to form an aqueous layer and an oily layer,
- (d) harvesting said aqueous layer to produce an extracted and concentrated antibody sample,
- (e) dialyzing said antibody sample, and
- (f) passing said dialysate over a chromatographic column to obtain a purified antibody preparation.
14-32. (canceled)
A method of extraction for a biological sample consisting essentially of:
- (a) adding at least one liquid carboxylic acid to a biological sample, to form a first composition,
- (b) mixing said first composition to form a second composition that contains an aqueous soluble product,
- (c) centrifuging said second composition to form an aqueous layer and an oily layer, and
- (d) harvesting said aqueous later which is a concentrated second sample selected from the group consisting of proteins, bacteria, virus, and mixtures thereof,
- wherein said aqueous layer which is a concentrated second sample is concentrated as compared to said biological sample.
37 A method for obtaining purified antibodies from a biological sample consisting essentially of:
- (a) adding at least one liquid carboxylic acid to an antibody containing biological sample to form a first composition,
- (b) mixing said first composition to form a second composition that contains an aqueous soluble product,
- (c) centrifuging said second composition to form an aqueous layer and an oily layer,
- (d) harvesting said aqueous layer which is an extracted and concentrated antibody containing second sample wherein said second sample is concentrated as compared to said antibody containing biological sample,
- (e) dialyzing said antibody containing sample, and
- (f) passing said dialysate over a chromatographic column to obtain a purified antibody preparation.
The method of claim 37 wherein said at least one liquid carboxylic acid is selected from the group consisting of caprylic acid, capric acid, oleic acid, isosteric acid, caproic acid, and mixtures thereof.
The method of claim 37 wherein said at least one liquid carboxylic acid is a mixture of two carboxylic acids.
Dependencies are inferred from the claim text.