Peptides for the detection of HIV-1 group O
OwnerRobert De Leys, Jian Zhenginventors
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Abstract
This invention relates to peptides and their preparation. The peptides each have a sequence that corresponds to the immunodominant region of the HIV-1 group O gp41 envelope protein. The sequence is characterized in that it does not correspond to any known naturally occurring group O sequence or variant. Furthermore, the peptide binds anti-HIV-1 group O antibodies. There are several uses for the peptides, including the detection of antibodies produced in response to HIV-1 group O infection. The peptides may also be incorporated in mosaics and expressed recombinantly.
Claims16
A peptide comprising an amino acid sequence selected from the group consisting of:
- (SEQ ID NO:59) NQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:61) EQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:60) XQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:62) ETLMQXQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:64) RARLQALETLMQNQQRLNSWGCKGRIICYTSARWH, (SEQ ID NO:65) DQQVNNVSSIIYDKILEAQDQQEENVRELLELD and functional derivatives thereof.
(canceled)
A host cell containing the expression vector of claim 8.
A test kit comprising one or more peptides selected from the group consisting of:
- (SEQ ID NO:59) NQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:61) EQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:60) XQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:62) ETLMQXQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:64) RARLQALETLMQNQQRLNSWGCKGRIICYTSARWH, (SEQ ID NO:65) DQQVNNVSSIIYDKILEAQDQQEENVRELLELD and, functional derivatives thereof, antibodies that bind to said peptides, and antibodies that bind to said functional derivatives thereof.
An in vitro diagnostic assay method comprising contacting a sample with one or more peptides selected from the group consisting of:
- (SEQ ID NO:59) NQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:61) EQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:60) XQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:62) ETLMQXQQRLNSWGCKGRIICYTSARWH,
- (SEQ ID NO:64) RARLQALETLMQNQQRLNSWGCKGRIICYTSARWH, (SEQ ID NO:65) DQQVNNVSSIIYDKILEAQDQQEENVRELLELD and, and functional derivatives thereof and determining binding between said peptide and an antibody.
A mosaic comprising a recombinant group M gp 41 protein wherein a group M immunodominant region has been replaced by one or more O-like immunodominant sequences.
A mosaic comprising a recombinant group M gp 41 protein wherein a group M immunodominant region has been replaced by one or more O-like immunodominant sequences wherein said mosaic is selected from the group consisting of:
- (SEQ ID NO:66)
- ARLLLSGIVQQQNNLLRAIEAQQHMLQLTAWGIKQLRARLQALETLMQNQ
- QRLNSWGCKGRIICYTSARWHASWSNKSLEDIWDNMTWMQWDQQVNNVSS
- IIYDKILEAQDQQEENVRELLELDKWASLWNWFDITNWLWYIKIFIIMIV
- GGLVGLRIVFAVLSIVNRVRQGYSPLSLQTRPPVPRGPDRPEGIEEEGGE
- RDRDTSGRLVHGFLAIIWVDL
- and
- (SEQ ID NO:67)
- ARLLLSGIVQQQNNLLRAIEAQQHMLQLTAWGIKQLRARLQALETLMQNQ
- QRLNSWGCKGRIICYTSARWHASWSNKSLEDIWDNMTWMQWDQQVNNVSS
- IIYDKILEAQDQQEENVRELLELDKWASLWNWFDITNWLWYIKIFIMIV.
Dependencies are inferred from the claim text.