Use of a virus expressing a binding moiety to measure analytes in a sample

A method of detecting an analyte in a sample, said method comprising contacting said sample with a virus, which expresses and displays a specific binding moiety, such that the virus forms a complex with the analyte or an analogue thereof, or a binding moiety for the analyte, and that the complex binds or does not bind a surface, depending upon the presence or absence of analyte in the sample, detecting the presence or absence of a nucleic acid on the surface using a nucleic acid amplification reaction, and relating that to the presence or absence of analyte in the sample.

A kit for detecting the presence of an analyte in a sample, said kit comprising solid body having immobilised on a surface thereof a binding reagent which either (a) binds said analyte, or (b) comprises said analyte or an analogue thereof, and a virus which expresses a specific binding moiety for either said analyte or said binding reagent in competition to said analyte.