Insecticidal extract from legume plants and method of preparing the same
AssigneeHer Majesty the Queen In Right of Canada, the Minister of Agriculture and Agri-Food
InventorsWesley Taylor, Paul Fields, Daniel Sutherland
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Abstract
The invention may be summarized as follows. The present invention provides insecticides comprised of naturally-occurring compounds, such as, for example, PA1b-related peptides and terpenoid saponins. The present invention also provides for identification and characterization of a synergistic insecticidal effect between peptides and saponins extracted from plants. Furthermore, simplified extraction procedures are provided that avoid the use of a chloroform defatting step or a column chromatography step.
Claims27
An insecticidal, alcohol-soluble extract of a non-defatted legume seed material, the extract comprising a PA1B-related peptide to saponin ratio ranging from 10:1 to 1:10, the PA1B-related peptide comprising an amino acid sequence of Cys-(Xaa)n-(Xaa)n-Cys-(Xaa)n-Pro-(Xaa)n-(Xaa)n-Pro-Cys-(Xaa)n-(Xaa)n-Cys-(Xaa)n-Cys-(Xaa)n-Pro-(Xaa)n-(Xaa)n-(Xaa)n-Cys (SEQ ID NO:63), where each Xaa is independently selected from any amino acid and each n independently equals 1, 2, 3, 4, or 5.
A method of preparing an insecticidal alcohol soluble legume extract comprising:
- extracting a non-defatted legume seed material with a C(1-4) alcohol.
An insecticidal composition comprising an isolated PA1b-related peptide and an isolated saponin, the peptide to saponin ratio ranging from 10:1 to 1:10, the PA1B-related peptide comprising an amino acid sequence of Cys-(Xaa)n-(Xaa)n-Cys-(Xaa)n-Pro-(Xaa)n-(Xaa)n-Pro-Cys-(Xaa)n-(Xaa)n-Cys-(Xaa)n-Cys-(Xaa)n-Pro-(Xaa)n-(Xaa)n-(Xaa)n-Cys (SEQ ID NO:63), where each Xaa is independently selected from any amino acid and each n independently equals 1, 2, 3, 4, or 5.
A method of controlling insects comprising exposing the insects to an effective amount of a composition comprising dehydrosoyasaponin I.
Dependencies are inferred from the claim text.