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US 2006/0216309 A1Published application
Identification of genes
AssigneeRPMS Technology Limited
InventorDavid Holden
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AssigneeRPMS Technology Limited
InventorDavid Holden
Estimating the term…
A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of: (1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism; (2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant; (3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment; (4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms; (5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marker sequence of each individual mutant stored as in step (2); and (6) selecting an individual mutant which does not contain any of the marker sequences as isolated in step (4).
A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of:
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A gene which is isolated from the Salmonella typhimurium genome and hybridizes to the sequence shown in FIG. 5 or FIG. 6 under stringent conditions, or a polypeptide encoded thereby.
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A molecule which selectively interacts with, and substantially inhibits the function of, a gene hybridizing under stringent conditions to the sequence of FIG. 5 or FIG. 6 or a nucleic acid product thereof.
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The VGC2 DNA of Salmonella typhimurium or a part thereof, or a variant of said DNA or a variant of a part thereof, or a polypeptide encoded thereby.
A mutant bacterium wherein if the bacterium normally contains a gene that is the same as or equivalent to a gene in VGC2, said gene is mutated or absent in said mutant bacterium.
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A method of identifying a compound which reduces the ability of a bacterium to infect or cause disease in a host comprising the step of selecting a compound which interferes with the function of a gene in the VGC2 DNA of Salmonella typhimurium or a part thereof, or a variant of said DNA or a variant of a part thereof or a polypeptide encoded thereby.
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A non-human animal or plant, or an animal or plant cell culture, containing a plurality of mutant microorganisms wherein each mutant contains a different marker sequence.
Dependencies are inferred from the claim text.