Solid phase extraction pipette
AssigneeVarian, Inc.
InventorsWilliam Hudson, Wilford Downs
Life of this patent
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Abstract
Solid phase extraction devices for sample preparation are disclosed, comprising a hollow conical tube having one narrower opening and one broader opening, wherein the narrower opening of the tube contains a solid phase extraction material comprising a functionalized monolithic sorbent, and wherein the solid phase extraction device is prepared by a combination of reduced pressure, positive pressure and mechanical compaction. The solid phase extraction devices are adapted for preparation of small sample volumes, and provide excellent recovery of analytes and good flow characteristics. Methods for preparing and using the solid phase extraction devices are disclosed.
Claims25
A solid phase extraction device for sample preparation, comprising a hollow conical tube having one narrower opening and one broader opening, wherein the narrower opening of the tube contains a solid phase extraction material comprising a functionalized monolithic sorbent, wherein the solid phase extraction device is prepared by a combination of reduced pressure, positive pressure and mechanical compaction.
A solid phase extraction pipette for sample preparation comprising a pipette having a smaller opening and a larger opening and a functionalized monolithic sorbent placed in the smaller opening of the pipette, wherein the functionalized monolithic sorbent is placed in the smaller opening of the pipette by a combination of reduced pressure, positive pressure and mechanical compaction.
A method for preparing a device for solid phase extraction, comprising the steps of:
- a) inserting a functionalized monolithic sorbent into a hollow tube having one broader opening and one narrower opening;
- b) applying reduced pressure to the narrower opening of the tube to insert the functionalized monolithic sorbent into the tube;
- c) applying positive pressure to the broader opening of the tube to insert the functionalized monolithic sorbent into the narrow opening of the tube;
- and
- d) compacting the functionalized monolithic sorbent.
A method for preparing a sample for analysis, comprising the steps of:
- a) activating a solid phase extraction pipette;
- b) adsorbing components of a sample to be analyzed onto the solid phase extraction pipette;
- c) washing the solid phase extraction pipette with a solvent which does not remove adsorbed analytes from the solid phase extraction material;
- d) washing the solid phase extraction pipette with a solvent that does remove adsorbed analytes from the solid phase extraction material;
- and
- e) collecting the analytes.
Dependencies are inferred from the claim text.