Narrow swab (access swab) for ATP Measurement
AssigneeNeogen Corporation
InventorsPaul Satoh, Donald Pielack, James Topper, Clifton Brown
Life of this patent
Estimating the term…
Abstract
A device and methods for the rapid chemiluminescence or calorimetric assay of surfaces to detect the presence of microbial or protein contamination is disclosed. A sampling/analysis member (10) is described having a sampling wand (15) which is suitable for use by untrained personnel under the relatively harsh and variable conditions found in the field, for example in fast food restaurants and other food preparation areas. The analytical signal in the disclosed device and methods can be based on luciferase/luciferin systems or a protein assay systems utilizing bicinchoninic acid.
Claims29
A sampling/analysis member which is used to assay for an analyte of interest in a sample comprising:
- (a) a sampling wand having a sampling swab at a proximal end for collecting the sample of the analyte of interest and a distal end for handling of the sampling wand;
- (b) a tubular container containing a rinsing solution mounted on the sampling wand with an open end containing a liquid water-insoluble polymer as a stopper for the solution adjacent the swab at the proximal end and a sealed end adjacent the distal end, wherein a portion of the tubular container intermediate the ends is frangible;
- and
- (c) an analysis structure for receiving the sample of the analyte of interest rinsed from the sampling swab by the rinsing solution upon rupture of the portion of the tubular container which is frangible and for retaining the analyte for the detection of the presence of the analyte of interest in the sample.
A sampling wand comprising:
- (a) a sampling swab at a proximal end for collecting a sample of an analyte of interest and a distal end for handling of the sampling wand;
- (b) a tubular container containing a solution mounted on the sampling wand with an open end containing a liquid water-insoluble polymer as a stopper for the solution adjacent the swab at the proximal end and a sealed end adjacent the distal end, wherein a portion of the tubular container intermediate the ends is frangible so as to release the solution and water-insoluble polymer into the sampling swab;
- and
- (c) a rupturing member on the sampling wand adjacent to the tubular member for rupturing the portion which is frangible.
A method for assaying for an analyte of interest in a sample which comprises:
- (a) providing a sampling wand at a proximal end for collecting a sample of an analyte of interest and a distal end for handling of the sampling wand;
- a tubular container containing a solution mounted on the sampling wand with an open end containing a liquid water-insoluble polymer as a stopper for the solution adjacent the swab at the proximal end and a sealed end adjacent the distal end, wherein a portion of the tubular container intermediate the ends is frangible so as to release the solution and water-insoluble polymer into the sampling swab;
- and a rupturing member on the sampling wand adjacent to the tubular member for rupturing the portion which is frangible;
- (b) rubbing the swab on the wand on a surface to retain any of the analyte on the swab;
- (c) inserting the sampling wand into an analysis structure for receiving the sample of interest;
- (d) rupturing the tubular container with the rupturing member so as to release the solution and the liquid water insoluble polymer into and through the swab into the analysis structure;
- and
- (e) detecting any of the analyte in the analysis structure.
A sampling wand comprising:
- (a) a sampling swab at a proximal end of the distal end of the wand for collecting a sample of an analyte of interest and having a distal end for handling of the sampling wand;
- and
- (b) a container containing a solution mounted on the sampling wand with an open end comprising a liquid water-insoluble polymer for the solution adjacent the swab at the proximal end and a sealed end adjacent the distal end, wherein a portion of the tubular container intermediate the ends is operable so as to release the solution and water-insoluble polymer into the sampling swab, thereby enabling the detection of the analyte on the swab in the solution.
Dependencies are inferred from the claim text.