Specimen filling device, specimen filling method, and liquid chromatography device with the specimen filling device

A sample injection method characterized by having

• a step of aspirating a sample into a sampling needle,
• a step of holding the sample in the sampling needle and dipping the sampling needle in cleaning liquid,
• a step of generating an ultrasonic wave in the cleaning liquid so as to clean the sampling needle dipped in the cleaning liquid, and
• a step of ejecting the sample from the sampling needle so as to inject the sample into a moving liquid.

A liquid chromatography apparatus having

• a mobile phase reservoir for storing a liquid as a mobile phase,
• a sample injection apparatus for injecting a sample into the liquid as a mobile phase,
• a separation column for separating a component of the sample sent from the sample injection apparatus and the liquid as a mobile phase, and
• a detector for detecting a component of the sample separated by the separation column,
• characterized in that the sample injection apparatus has a sampling vessel into which a sample is supplied, a sampling needle for aspirating and ejecting the sample, a cleaning part into which a cleaning liquid for cleaning at least the sampling needle is supplied, a sample injection part for injecting the sample ejected from the sampling needle into the liquid as a mobile phase, and needle transfer means for transferring the sampling needle among the sampling vessel, the cleaning part and the sample injection part, wherein
• the cleaning part has an ultrasonic vibrator for generating an ultrasonic wave in the cleaning liquid.