Preparation of deallergenized proteins and permuteins

A method of preparing a recombinant host cell, the method comprising:

• selecting a host cell;
• transforming the host cell with a recombinant vector;
• and
• obtaining recombinant host cells;
• wherein the recombinant vector comprises a structural nucleic acid sequence encoding a protein selected from the group consisting of:
• (amino acids 24-246 of SEQ ID NO:2)-linker-(amino acids 247-386 of SEQ ID NO:2);
• and
• (amino acids 24-268 of SEQ ID NO:2)-linker-(amino acids 269-386 of SEQ ID NO:2).

A permutein protein comprising:

• (amino acids 24-246 of SEQ ID NO:2)-linker-(amino acids 247-386 of SEQ ID NO:2) or (amino acids 24-268 of SEQ ID NO:2)-linker-(amino acids 269-386 of SEQ ID NO:2);
• the permutein protein modified by replacing one or more amino acids in the following regions with alanine, glutamic acid, phenylalanine, proline, serine, or glutamine:
• positions 104-113 of SEQ ID NO:2;
• positions 128-137 of SEQ ID NO:2;
• positions 184-197 of SEQ ID NO:2;
• position 202 of SEQ ID NO:2;
• positions 264-277 of SEQ ID NO:2;
• positions 316-325 of SEQ ID NO:2;
• and
• positions 360-377 of SEQ ID NO:2;
• wherein the permutein protein displays reduced binding to anti-acyl lipid hydrolase antibodies with respect to the binding of unmodified acyl lipid hydrolase protein to the anti-acyl lipid hydrolase antibodies.

An isolated nucleic acid molecule segment comprising a structural nucleic acid sequence, the structural nucleic acid sequence encoding a permutein protein selected from the group consisting of (amino acids 24-246 of SEQ ID NO:2)-linker-(amino acids 247-386 of SEQ ID NO:2) and (amino acids 24-268 of SEQ ID NO:2)-linker-(amino acids 269-386 of SEQ ID NO:2);

• the permutein protein modified by replacing one or more amino acids in the following regions with alanine, glutamic acid, phenylalanine, proline, serine, or glutamine:
• positions 104-113 of SEQ ID NO:2;
• positions 128-137 of SEQ ID NO:2;
• positions 184-197 of SEQ ID NO:2;
• position 202 of SEQ ID NO:2;
• positions 264-277 of SEQ ID NO:2;
• positions 316-325 of SEQ ID NO:2;
• and
• positions 360-377 of SEQ ID NO:2;
• wherein the permutein protein displays reduced binding to anti-acyl lipid hydrolase antibodies with respect to the binding of unmodified acyl lipid hydrolase protein to the anti-acyl lipid hydrolase antibodies.