Binding assay using binding agents with tail groups

A method of preparing a solid support having a plurality of different species of binding agents immobilized thereon at discrete locations, wherein each species of binding agent is an antibody capable of binding to a given analyte and is attached to an oligonucleotide tail group capable of hybridizing to a capture agent, said method comprising:

• a) immobilizing a plurality of capture agents at discrete locations on the solid support, wherein the different species of capture agent comprise different oligonucleotides having sequences that are capable of hybridizing to the oligonucleotide tail group of a given binding agent;
• and,
• b) contacting the binding agents with the solid support having plurality of capture agents immobilized thereon so that the binding agents become specifically bound to their respective capture agents by the hybridisation of the plurality of different capture agents to the complementary sequences of the tail groups of their corresponding binding agent.

A solid support for use in determining the concentration of one or more analytes in a liquid sample, the solid support having immobilized thereon a plurality of different species of binding agents, wherein each species of binding agent is an antibody capable of binding a given analyte and has a tail group, the tail groups of each different species of binding agent having nucleotide sequences that are different from one another, said plurality of different species of binding agents being specifically attached to the support by a plurality of capture agents at discrete locations on said support, wherein the capture agents at each discrete location are oligonucleotides having common sequences which are hybridized to a complementary sequence of a tail group of a corresponding binding agent from among said plurality of different species of binding agent.