Storage stable frozen lactic acid bacteria culture
OwnerHans Bisgaard-Frantzen, Borge Kringelum +1inventors
InventorsHans Bisgaard-Frantzen, Borge Kringelum, Inge Knap
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Abstract
A storage stable frozen lactic acid bacteria (LAB) culture that comprises LAB that are that are able to utilize sucrose, has a weight of at least 50 g frozen material and a content of viable bacteria of at least 109 colony forming units (CFU) per g frozen material.
Claims20
A frozen lactic acid bacteria (LAB) culture that comprises LAB that are able to utilize sucrose and is a mixed mesophilic culture consisting of mesophilic bacteria having optimum growth temperatures at about 30° C., has a weight of at least 50 g frozen material and a content of viable bacteria of at least 109 colony forming units (CFU) per g frozen material and characterized in that the frozen culture comprises from 0.5% to 80% of a cryoprotective agent measured as w/w of the frozen material.
A method for making a frozen lactic acid bacteria (LAB) culture that comprises LAB that are able to utilize sucrose, has a weight of at least 50 g frozen material and a content of viable bacteria of at least 109 colony forming units (CFU) per g frozen material comprising following steps:
- (i) adding a cryoprotective agent to viable bacteria to get at least 50 g of material with a content of viable bacteria of at least 109 colony forming units (CFU) per g material and comprising the cryoprotective agent in an amount from 0.5% to 80% measured as w/w of the material,
- (ii) freezing the material to get frozen material, and
- (iii) packing the frozen material in a suitable way.
A commercially viable, highly concentrated lactic acid bacteria (LAB) culture adapted to be frozen, the culture comprising:
- lactic acid bacteria (LAB) of at least 1×109 colony forming units (CFU) per gram of culture;
- and,
- a cryoprotective agent;
- wherein the cryoprotective agent is 0.5% to 80% (w/w) of the culture;
- wherein the culture has a weight of at least 50 grams;
- and,
- wherein the LAB are adapted to utilize sucrose;
- and are mesophilic having optimum growth temperatures of about 30° C.
Dependencies are inferred from the claim text.