DWF4 polynucleotides, polypeptides and uses thereof
AssigneeThe Arizona Board of Regents on Behalf of the University of Arizona, a Arizona corporation
InventorsRicardo Azpiroz, Sunghwa Choe, Kenneth Feldmann
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Abstract
The present invention relates to novel, polynucleotides isolated from dwarf plants. The dwf4 polynucleotides that encode all, or a portion of, a DWF4 polypeptide, a cytochrome P450 enzyme that mediates multiple steps in synthesis of brassinosteriods. The present invention also relates to isolated polynucleotides that encode regulatory regions of dwf4. Uses of the dwf4 polypeptides and polynucleotides are also disclosed.
Claims18
1-57. (canceled)
An isolated polynucleotide comprising a nucleic acid encoding a polypeptide having at least about 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein said polypeptide contains a P450 A domain, a P450 B domain, a P450 C domain, and a P450 D domain, and wherein said polypeptide is effective for catalysing the hydroxylation of a compound selected from the group consisting of campestanol and 6-oxocampestanol.
A transgenic plant containing at least one exogenous polynucleotide, said at least one exogenous polynucleotide comprising a control element operably linked to a nucleic acid encoding a polypeptide having at least about 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein said polypeptide contains a P450 A domain, a P450 B domain, a P450 C domain, and a P450 D domain, and wherein said polypeptide is effective for catalysing the hydroxylation of a compound selected from the group consisting of campestanol and 6-oxocampestanol.
A method of making a transgenic plant comprising introducing into a plant a polynucleotide comprising a control element operably linked to a nucleic acid encoding a polypeptide having at least about 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein said polypeptide contains a P450 A domain, a P450 B domain, a P450 C domain, and a P450 D domain, and wherein said polypeptide is effective for catalysing the hydroxylation of a compound selected from the group consisting of campestanol and 6-oxocampestanol.
An isolated polynucleotide comprising nucleotides 2102 to 3202 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.