Plant transcription factor that is involved in sugar signalling
OwnerChrister Jansson, Chuanxin Suninventors
Life of this patent
Estimating the term…
Abstract
Provided are nucleotide sequences which encodes a sugar-signalling transcription factors capable of activating a promoter of a gene encoding an enzyme involved in the synthesis or deposition of starch e.g. in response to sugar levels in a plant. Preferred sequences encode WRKY proteins such as the sugar signaling in barley2 (SUSIBA2) factor and variants thereof, and act on promoters which comprise at least one SURE element and\or W box element to which the transcription factor binds (e.g. promoters such as iso1, sbe1, sbeIIb, ssI, agpaseS). The invention also provides related methods and materials e.g. for activating, modulating, and investigating the elements present in such promoters.
Claims34
An isolated nucleic acid which comprises a nucleotide sequence which encodes a sugar-signalling transcription factor which is capable of activating a promoter of a gene encoding an enzyme involved in the synthesis or deposition of starch.
An isolated nucleic acid for use as a probe or primer, said nucleic acid having a distinctive sequence of at least about 16-24 nucleotides in length, which sequence is present in FIG. 1 or a sequence which is degeneratively equivalent thereto, or the complement of either.
(canceled)
A method of investigating or confirming whether a cis promoter element is present in a plant transcription factor consensus sequence in a target gene promoter, the method comprising:
- (i) observing the expression of a reporter gene operably linked to the promoter in a plant cell in which the transcription factor is present,
- (ii) introducing into the plant cell a double stranded oligodeoxynucleotide (ODN) decoy corresponding to the promoter element into the cell,
- (iii) observing the expression of the reporter gene in the presence of the ODN decoy,
- wherein a reduction in expression from (i) to (iii) confirms that the plant transcription factor binds the promoter element.
34-37. (canceled)
Dependencies are inferred from the claim text.