Automated method for high throughput screening of nematodes
OwnerMatthew Gill, Anders Olsen +1inventors
InventorsMatthew Gill, Anders Olsen, Gordon Lithgow
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Abstract
This invention provides a high throughput survival assay, using uptake of a marker dye (e.g. a fluorescent dye) as a marker of death of a nematode. The assay permits high throughput screening of thousands of compounds possible. By the application of automated worm handling technology we are able to accurately dispense nematodes into 384 well microtitre plates, at rates many thousand of Limes faster than previously possible. In addition, we have automated the analysis of survival by the use of a fluorometric plate reader that quantitates the degree of fluorescence within each well.
Claims65
A method for screening for the activity of one or more agents on a nematode, said method comprising:
- providing a microtiter plate comprising a plurality of wells, each of a plurality of said wells containing one or more nematodes;
- contacting said nematodes with one or more test agents;
- contacting said nematodes with a detectable label that indicates the viability of said nematodes;
- and
- detecting said detectable label to ascertain the viability of nematodes contacted with said one or more test agents where an increase or decrease in the viability of the nematodes contacted with said test agents that the nematodes are susceptible to the activity of said test agent or that said test agent is active on said nematodes.
A device for screening for the activity of one or more agents on a nematode, said device comprising:
- a microtiter plate comprising a plurality of wells, each of a plurality of said wells containing one or more nematodes.
A kit for screening for the activity of one or more agents on a nematode, said kit comprising:
- a microtiter plate comprising a plurality of wells;
- a plurality of nematodes;
- and
- a detectable label that indicates the viability of said nematodes.
Dependencies are inferred from the claim text.