Databases for assessing nucleic acids
AssigneesMedical College of Ohio, Gene Express, Inc.
InventorsJames Willey, Brad Austermiller, Erin Crawford, Charles Knight, Terry Osborn, Robert Zahorchak
Life of this patent
Estimating the term…
Abstract
The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.
Claims34
A database comprising numerical values corresponding to amounts of a first nucleic acid in a number of samples wherein said numerical values are directly comparable between about 5 of said samples.
A method for obtaining a numerical index that indicates a biological state, comprising:
- providing 2 samples corresponding to each of a first biological state and a second biological state;
- assessing an amount of each of 2 nucleic acids in each of said 2 samples wherein said assessing can enumerate less than about 1,000 molecules of each of said 2 nucleic acids;
- providing said amounts as numerical values wherein said numerical values are directly comparable between a number of samples;
- mathematically computing said numerical values corresponding to each of said first and said second biological states;
- and
- determining a mathematical computation that discriminates said first and said second biological states, thereby obtaining said numerical index.
A method comprising:
- using a microarray to identify 2 nucleic acids associated with a first biological state and not with a second biological state;
- providing 2 samples corresponding to each of said first biological state and said second biological state;
- assessing an amount of each of said 2 nucleic acids in each of said 2 samples wherein said assessing can enumerate less than about 1,000 molecules of each of said 2 nucleic acids;
- and
- mathematically computing said amounts corresponding to each of said first and said second biological states to determine a numerical index, said numerical index discriminating said first and said second biological states.
A method of identifying a biological state comprising:
- assessing an amount each of 2 nucleic acids in a first sample, wherein said assessing can enumerate less than about 1,000 molecules of each of said 2 nucleic acids in said first sample;
- providing said amounts as numerical values wherein said numerical values are directly comparable between a number of samples;
- and
- using said numerical values to provide a numerical index, whereby said numerical index indicates said biological state.
A method of identifying a biological state comprising:
- assessing an amount a nucleic acid in a first sample, wherein said assessing can enumerate less than about 1,000 molecules of said nucleic acid in said first sample;
- and
- providing said amount as a numerical value wherein said numerical value is directly comparable between a number of other samples.
Dependencies are inferred from the claim text.