Use of ph-responsive polymers
OwnerJames Van Alstine, Camilla Larsson +2inventors
InventorsJames Van Alstine, Camilla Larsson, Ronnie Palmgren, Asa Rudstedt
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Abstract
The present invention relates to a method of isolating target compounds from a liquid, which comprises at a first pH, contacting the liquid with a separation medium that exhibits surface-localised pH-responsive polymers in to adsorb the target compound via hydrophobic interactions; and adding an eluent, which is of a second pH and provides a conformational change of said pH-responsive polymers to release said compounds. The elution is advantageously performed by a pH gradient and/or by a salt gradient.
Claims19
A method of isolating at least one target compound from a liquid, which method comprises the steps of
- (a) contacting the liquid, at a first pH, with a separation medium that includes surface-localised pH-responsive polymers to adsorb the target compound(s) via hydrophobic interactions;
- and
- (b) adding an eluent of a second pH value, which eluent provides a conformational change of said pH-responsive polymers, to release the target compound(s) from the separation medium.
15-18. (canceled)
A hydrophobic interaction chromatography (HIC) medium, which is comprised of a matrix to which surface-localised pH-responsive polymers have been attached, which polymers include HIC ligands.
A kit for isolating target compounds, which kit comprises, in separate compartments, a chromatography column packed with a medium comprised of a matrix to which surface-localised pH-responsive polymers, which exhibit HIC ligands, have been attached;
- an adsorption buffer of a first pH;
- and an eluent of a second pH, which is lower that said first pH.
Dependencies are inferred from the claim text.