Molecular method to augment RNA mediated gene silencing
OwnerAjit Kumarinventor
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Abstract
The invention provides for a novel method of augmenting gene silencing via RNA interference (RNAi). Under the invention, RNAi technology is combined with the action of a variant nuclear factor to potently inhibit gene expression. In one embodiment of the invention, the nuclear factor is a variant of a double-stranded RNA (dsRNA) binding protein termed NF90ctv. The invention is also related to diagnostic/investigative and treatment methods and to cell lines produced by the methods disclosed.
Claims6
A method for augmenting the silencing of a target gene in a cell comprising:
- Providing a short interfering RNA (siRNA) specific for said target gene, and an enhancing agent;
- Introducing said siRNA into said cell;
- and
- Introducing an effective amount of said enhancing agent into said cell.
A method for augmenting the silencing of a target gene in a cell comprising:
- Providing a short interfering RNA (siRNA) specific for said target gene, and an expression vector encoding an enhancing agent;
- Introducing said siRNA and said vector into said cell.
A kit for augmenting the silencing of a target gene in a cell comprising;
- A short interfering RNA (siRNA) specific for said target gene;
- and,
- An enhancing agent.
Dependencies are inferred from the claim text.