Delivery of substances to cells
AssigneePhogen Limited
InventorsPeter Francis O'Hare, Neil Brewis, Nadia Normand, Kavitha Sunassee
Life of this patent
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Abstract
Aggregates comprising VP22 protein and oligonucleotides or polynucleotides can be used together with a disaggregating agent (simultaneously or sequentially) to treat target cells by delivery of molecules to the cells and/or to prevent cell proliferation and/or to kill cells.
Claims21
A method of treating target cells to deliver molecules to said cells, comprising
- (a) exposing the cells to an aggregate composition which comprises VP22, or a polypeptide with the transport function of VP22, and oligonucleotides or polynucleotides;
- and
- (b) exposing the cells to a disaggregating agent which can promote disaggregation of the aggregate composition in cells,
- wherein steps (a) and (b) are carried out either simultaneously or sequentially, thereby delivering the molecules to the cells.
A product comprising (a) an aggregate composition which comprises VP22, or a polypeptide with the transport function of VP22, and oligonucleotides or polynucleotides, and (b) a disaggregating agent which can promote disaggregation of the aggregate composition in cells, as a combined preparation for administration of the components (a) and (b) either sequentially or together, for use in treating cells by delivery of molecules to cells.
A pharmaceutical comprising
- (a) an aggregate composition which comprises VP22, or a polypeptide with the transport function of VP22, and oligonucleotides or polynucleotides;
- and
- (b) a disaggregating agent which can promote disaggregation of the aggregate composition in cells,
- in combination with a pharmaceutically acceptable excipient.
Dependencies are inferred from the claim text.