Peptides and nucleic acids of the cathelicidin family, derived from fish, and uses thereof
AssigneeThe University Court of the University of Aberdeen
InventorsChristopher Secombes, Olga Pleguezuelos
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Abstract
The invention relates to anti-microbial and immunostimulatory molecules of the cathelicidin family derived from fish. The invention provides a novel cathelicidin molecule, fragments, derivatives and uses thereof, and nucleic acids encoding the same.
Claims52
An isolated polypeptide having anti-microbial activity, comprising at least one repeat of the amino acid sequence RPG-G/V-GS-X-I/P-G (SEQ ID NO:
- 19), said polypeptide optionally comprising a pair of cysteine residues capable of forming an internal disulphide bond.
A polypeptide selected from the group consisting of at least one repeat of the amino acid sequence RPG-G/V-GS-X-I/P-G (SEQ ID NO:
- 19), SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:16 SEQ ID NO:
- 18, wherein said polypeptide optionally comprises a pair of cysteine residues capable of forming an internal disulphide bridge, said polypeptide, having anti-fungal or anti-bacterial activity.
An isolated polypeptide, comprising a cathelin signature sequence comprising the amino acid sequence of SEQ ID NO:
- 6, 8 or 25.
An antibody specific for a polypeptide selected from the group consisting of at least one repeat of the amino acid sequence RPG-G/V-GS-X-I/P-G (SEQ ID NO:
- 19), SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:16, SEQ ID NO:
- 18, SEQ ID NO:
- 10, SEQ ID NO:
- 27, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 25, SEQ ID NO:
- 4, SEQ ID NO:
- 23, wherein said polypeptide optionally comprises a pair of cysteine residues capable of forming an internal disulphide bridge and has anti-fungal or anti-bacterial activity.
An isolated nucleic acid encoding a polypeptide selected from the group consisting of at least one repeat of the amino acid sequence RPG-G/V-GS-X-I/P-G (SEQ ID NO:
- 19), SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:16, SEQ ID NO:
- 18, SEQ ID NO:
- 10, SEQ ID NO:
- 27, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 25, SEQ ID NO:
- 4, and SEQ ID NO:
- 23.
A nucleic acid primer or probe, comprising at least 16 to 24 contiguous nucleotides of a sequence selected from the group of sequences shown in SEQ ID NO:
- 1, SEQ ID NO:
- 20, and FIG. 15, or the complement thereof, or a sequence degenerately equivalent thereto.
A method for isolating a nucleic acid encoding a cathelicidin polypeptide or a portion thereof, said method employing a nucleic acid primer or probe sequence selected from the group consisting of a sequence comprising at least 16 to 24 contiguous nucleotides of SEQ ID NO:
- 1, SEQ ID NO:
- 20, sequences shown in FIG. 15, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:15, SEQ ID NO:
- 17, SEQ ID NO:
- 19, SEQ ID NO:
- 3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:22, SEQ ID NO:24 and SEQ ID NO:
- 26, the complement thereof, or a sequence degenerately equivalent thereto.
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Dependencies are inferred from the claim text.