Peptides for detection of antibody to Ehrlichia ewingii
AssigneeIDEXX Laboratories, Inc.
InventorThomas O'Connor
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Abstract
The invention provides compositions and methods for the detection and quantification of Ehrlichia ewingii, Ehrlichia ewingii antibodies, antibody fragments, and polypeptides.
Claims34
A composition of matter comprising a purified polypeptide consisting essentially of SEQ ID NO:3.
A purified fusion polypeptide comprising polypeptides of SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or a combination thereof.
A method of detecting antibodies that specifically bind E. ewingii or an E. ewingii polypeptide comprising:
- (a) contacting a purified polypeptide comprising SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or a combination thereof, with a test sample suspected of comprising antibodies specific for E. ewingii, under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies specific for E. ewingii are present in the test sample, and wherein the absence of polypeptide/antibody complexes is an indication that antibodies specific for E. ewingii are not present in the test sample.
A method of detecting an E. ewingii infection in a mammal comprising:
- (a) obtaining a biological sample from a mammal suspected of having an E. ewingii infection;
- (b) contacting a purified polypeptide comprising SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or a combination thereof with the biological sample under conditions that allow polypeptide/antibody complexes to form;
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that the mammal has an E. ewingii infection and wherein the absence of polypeptide/antibody complexes is an indication that the mammal does not have an E. ewingii infection.
An antibody that specifically binds to at least one epitope of an E. ewingii polypeptide, wherein said polypeptide is SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4.
A method of detecting an E. ewingii polypeptide or E. ewingii in a sample comprising:
- (b) contacting one or more antibodies that specifically bind to at least one epitope of an E. ewingii polypeptide, wherein said polypeptide comprises SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, or SEQ ID NO:4 with the sample under conditions that allow polypeptide/antibody complexes to form;
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that E. ewingii or an E. ewingii polypeptide is present in the sample and the absence of polypeptide/antibody complexes is an indication that E. ewingii or an E. ewingii polypeptide is not present in the sample.
Dependencies are inferred from the claim text.