Electroporation method including the use of depressurization/pressurization

An electroporation apparatus for transferring a nucleic acid into a cell, in combination with holding the cell under a pressure different from an atmospheric pressure, the apparatus comprising:

• a first electrode functioning as an anode;
• and
• a second electrode functioning as a cathode,
• wherein a distance between the first electrode and the second electrode is long enough to accommodate a plant seed.

An electroporation apparatus for transferring a nucleic acid into a cell, in combination with holding the cell under a pressure different from an atmospheric pressure, the apparatus comprising:

• a first electrode functioning as an anode;
• and
• a second electrode functioning as a cathode,
• wherein a distance between the first electrode and the second electrode can be changed so that a plant seed can be accommodated between the first and second electrodes.

An apparatus for automatically performing electroporation, comprising:

• a) a container for placing a mixture containing a nucleic acid and cells;
• b) a section for placing the nucleic acid in container a);
• c) a section for placing the cells in container a);
• d) a container for holding the cells under a pressure different from an atmospheric pressure, the container being capable of resisting the pressure different from the atmospheric pressure;
• e) a section for placing the cells in container d);
• f) a section for maintaining the inner area of container d) at the pressure different from the atmospheric pressure;
• g) a container for applying a high voltage pulse to the mixture containing the nucleic acid and the cells;
• h) a section for placing the mixture containing the nucleic acid and the cells in container g);
• i) a section for applying a high voltage pulse to the mixture containing the nucleic acid and the cells in container g);
• and
• j) a section for automatically performing section b), c), e), f), h), and i),
• wherein section b) and section c) are the same or different from each other;
• section e) and section h) are the same or different from each other;
• and container a), container d), and container g) are the same or different from one another.