US 2006/0188982 A1 · Multiplexed analysis for determining a serodiagnosis of viral infection

Abstract

Clinical samples can be analyzed using microparticles to determine the serodiagnosis of a viral infection from two candidate viral infections of the same viral group. Serodiagnosis can be determined via a pooled population of subsets of microparticles, with the particles in the pooled population having a bound viral group-reactive antibody and the particles in each subset having at least one characteristic classification parameter that distinguishes between subsets. Viral antigens of antibodies of interest in the same viral-class as the viral group-reactive antibody can be bound to the viral group-reactive antibody on the microparticles, and subsequently exposed to a clinical sample. Binding and labeling can be used. Automated analysis of data from multiplexed flow analysis can determine the presence or absence of antibodies of interest in the sample, thereby diagnosing for two candidate viral infections in a single assay.

Claims39

Dependent claims

A method for determining presence or absence of antibodies of interest in a sample with a set of microparticles to which a viral group-reactive antibody is covalently bound, the method comprising:

mixing a first mixture comprising:
(a) the sample,
(b) a first subset of the set of microparticles comprising a first reactive microparticle type comprising a first specific viral antigen reactive with the viral group-reactive antibody and a first specific viral antibody of interest in the sample, and
(c) a second subset of the set of microparticles comprising a second reactive microparticle type comprising a second specific viral antigen reactive with the viral group-reactive antibody and a second specific viral antibody of interest in the sample;
mixing a second mixture comprising the first mixture and a labeled reagent reactive with the antibodies of interest;
and
analyzing the set of microparticles in the second mixture to establish the presence or absence of the first and second specific viral antibodies.

4 more elements

Dependencies are inferred from the claim text.

Multiplexed analysis for determining a serodiagnosis of viral infection

Life of this patent

Multiplexed analysis for determining a serodiagnosis of viral infection

Life of this patent

Abstract

Claims39