US 2006/0188976 A1Published application

Mutant protein having the peptide-synthesizing activity

AssigneeAjinomoto Co., Inc.

InventorsRie Takeshita, Isao Abe, Masakazu Sugiyama, Kenzo Yokozeki, Seiichi Hara, Sonoko Suzuki, Shunichi Suzuki, Kunihiko Watanabe

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Abstract

The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant protein having the amino acid sequence containing one or more mutations of the above mutations 1 to 38 in the amino acid sequence described in SEQ ID NO:2; and (II) The mutant protein having the amino acid sequence containing one or more mutations selected from the group consisting of substitution, deletion, insertion, addition and inversion at positions other than one or more mutation positions of the above mutation 1 to 38 in the mutant protein described in the above (I), and having a peptide-synthesizing activity.

Claims9

Dependent claims

A mutant protein having an amino acid sequence comprising one or two or more mutations selected from any of the following mutations 1 to 38:

mutation 1:
F207V,
mutation 2:
Q441E,
mutation 3:
K83A,
mutation 4:
A301V,
mutation 5:
V257I,
mutation 6:
A537G,
mutation 7:
A324V,
mutation 8:
N607K,
mutation 9:
D313E,
mutation 10:
Q229H,
mutation 11:
M208A,
mutation 12:
E551K,
mutation 13:
F207H,
mutation 14:
T72A,
mutation 15:
A137S,
mutation 16:
L439V,
mutation 17:
G226S,
mutation 18:
D619E,
mutation 19:
Y339H,
mutation 20:
W327G,
mutation 21:
V184A,
mutation 22:
V184C,
mutation 23:
V184G,
mutation 24:
V184I,
mutation 25:
V184L,
mutation 26:
V184M,
mutation 27:
V184P,
mutation 28:
V184S,
mutation 29:
V184T,
mutation 30:
Q441K,
mutation 31:
N442K,
mutation 32:
D203N,
mutation 33:
D203S,
mutation 34:
F207A,
mutation 35:
F207S,
mutation 36:
Q441N,
mutation 37:
F207T, and
mutation 38:
F207I in an amino acid sequence of SEQ ID NO:2.

73 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/311,467Filed 20 Dec 2005
Priority: JP 2004-36850320 Dec 2004
Published: 24 Aug 2006
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C07K 14/00
Peptides having more than 20 amino acids
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria
C12N 9/93
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: ligases
C07K
Peptides