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US 2006/0188966 A1Published application
Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
OwnerPaul DeAngelisinventor
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Abstract
The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate or chondroitin or heparin/heparosan synthases from Pasteurella multocida, in order to create a variety of glycosaminoglycan oligosaccharides having a natural or chimeric or hybrid sugar structure.
Claims11
1-172. (canceled)
A method for producing a chimeric or hybrid glycosaminoglycan having a non-natural structure, comprising the steps of:
- providing a functional acceptor, wherein the functional acceptor has at least two sugar units selected from the group consisting of uronic acid and hexosamine;
- providing at least one glycosaminoglycan transferase, wherein the glycosaminoglycan transferase is selected from the group consisting of pmHAS, pmCS, pmHS, PglA, and mutants thereof;
- and
- providing at least one of UDP-GlcUA, UDP-GlcNAc, UDP-Glc and UDP-GalNAc such that the at least one glycosaminoglycan transferase elongates the functional acceptor in a manner so as to provide a chimeric or hybrid glycosaminoglycan having a non-natural structure.
1 more element
A library of natural glycosaminoglycan polymers produced according to the process comprising the steps of:
- treating at least one functional acceptor with at least one glycosaminoglycan transferase in the presence of at least one UDP-sugar selected from the group consisting of UDP-GlcNAc, UDP-GlcUA, UDP-GalNAc, and UDP-GlcN.
182-187. (canceled)
Dependencies are inferred from the claim text.