Reagent for producing a protein chip
OwnerYaeta Endo, Tatsuya Sawasakiinventors
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Abstract
An object of the present invention is to provide a reagent for protein synthesis by a simple operation which is advantageous in a cell-free protein synthesis in many samples in a test using an aimed (specific) protein or a high throughput analysis using a protein chip, etc. and also to provide a method for the synthesis of protein using the same. Further objects are to provide a protein library using the above-mentioned reagent, a kit for preparing a protein chip, a kit for testing an aimed (specific) protein, etc.
Claims15
A protein chip reagent utilizing a cell-free protein synthesis system which comprises the following elements:
- a:
- a solution containing cell extract for a cell-free protein synthesis comprising a wheat embryo extract wherefrom endosperm components and low-molecular protein synthesis inhibitors are substantially removed is added to each different well of a container which is partitioned in plural sections;
- b:
- at least the substances necessary for protein synthesis containing substrate and energy source and a specific translation template are added to the well mentioned in “a”;
- and
- c:
- the solution in the well mentioned in “b” is freeze-dried preparation (by freeze-drying).
A protein chip reagent utilizing a cell-free protein synthesis system which comprises the following elements:
- a:
- a solution containing cell extract for a cell-free protein synthesis comprising a wheat embryo extract wherefrom endosperm components and low-molecular protein synthesis inhibitors are substantially removed is added to each different well of a container which is partitioned in plural sections;
- b:
- at least the substances necessary for protein synthesis containing substrate and energy source and a specific translation template are added to the well mentioned in “a”;
- c:
- the solution in the well mentioned in “b” is freeze-dried preparation (by freeze-drying);
- and
- d:
- amount of a deliquescent substance in the freeze-dried preparation in the well mentioned in “c” is 0.01 part by weight or less to 1 part by weight of the protein in the said freeze-dried preparation.
A protein chip reagent utilizing a cell-free protein synthesis system which comprises the following elements:
- a:
- a solution containing cell extract for a cell-free protein synthesis comprising a wheat embryo extract wherefrom endosperm components and low-molecular protein synthesis inhibitors are substantially removed is added to each different well of a container which is partitioned in plural sections;
- b:
- at least the substances necessary for protein synthesis containing substrate and energy source and a specific translation template are added to the well mentioned in “a”;
- c:
- the solution in the well mentioned in “b” is freeze-dried preparation (by freeze-drying);
- d:
- amount of a deliquescent substance in the freeze-dried preparation in the well mentioned in “c” is 0.01 part by weight or less to 1 part by weight of the protein in the said freeze-dried preparation;
- and
- e:
- different kind type of translation template is contained in each the solution mentioned in “b” and makes two or more kinds of proteins synthesizable in each different well of the container which is partitioned in plural sections.
A protein chip reagent utilizing a cell-free protein synthesis system which comprises the following elements:
- a:
- a solution containing cell extract for a cell-free protein synthesis comprising a wheat embryo extract wherefrom endosperm components and low-molecular protein synthesis inhibitors are substantially removed is added to each different well of a container which is partitioned in plural sections;
- b:
- at least the substances necessary for protein synthesis containing substrate and energy source and a specific translation template are added to the well mentioned in “a”;
- c:
- the solution in the well mentioned in “b” is freeze-dried preparation (by freeze-drying);
- d:
- amount of a deliquescent substance in the freeze-dried preparation in the well mentioned in “c” is 0.01 part by weight or less to 1 part by weight of the protein in the said freeze-dried preparation;
- e:
- different kind type of translation template is contained in each the solution mentioned in “b” and makes two or more kinds of proteins synthesizable in each different well of the container which is partitioned in plural sections;
- and
- f:
- protein synthesized from the translation template is modified for fixation and is also coated with a substance having affinity to a substance added by the said modification for fixation to a surface in the well and/or a carrier in the well.
Dependencies are inferred from the claim text.