Screening methods
OwnerVendela Parrow, Lars Bergquist +2inventors
InventorsVendela Parrow, Lars Bergquist, Catrine Dreifeldt, Christine Flodin
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Abstract
The present invention relates to methods for the identification of modulators of cytokine class I receptors by determining whether a compound that binds to a cytokine class I receptor at a site different from the binding site of the naturally-occurring cytokine ligand is effective at modulating the amount of the cytokine class I receptor on the surface of the cell.
Claims35
A method of characterizing the bioactivity of a compound that binds to a cytokine class I receptor, the method comprising:
- providing a cell expressing a cytokine class I receptor on its cell surface;
- contacting the cell with a compound that binds to the cytokine class I receptor at a site different from the binding site of the naturally-occurring cytokine ligand;
- and
- determining whether the compound modulates the amount of the cytokine class I receptor on the surface of the cell.
A method of identifying a modulator of a cytokine class I receptor, the method comprising:
- screening to identify a compound that binds to a cytokine class I receptor at a site different from the binding site of the naturally-occurring cytokine ligand;
- contacting a cell expressing the cytokine class I receptor on its cell surface with the compound;
- and
- determining whether the compound modulates the amount of the cytokine class I receptor on the surface of the cell.
A method for determining the number of cells in a cell sample, the method comprising:
- providing a cell sample immobilized on a solid surface;
- contacting the cell sample with a fluorescent DNA stain comprising Vistra Green;
- incubating the cell sample in the presence of the fluorescent DNA stain;
- measuring the amount of fluorescence emitted by the cell sample;
- and
- comparing the measured fluorescence to a standard curve to determine the number of cells present in the cell sample.
Dependencies are inferred from the claim text.