Color-encoding and in-situ interrogation of matrix-coupled chemical compounds
AssigneesBioArray Solutions, Ltd., Rutgers, The State University of New Jersey
InventorsMichael Seul, Richard Ebright
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Abstract
A method and apparatus for the physico-chemical encoding of a collection of beaded resin (“beads”) to determine the chemical identity of bead-anchored compounds by in-situ interrogation of individual beads. The present invention provides method and apparatus to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial compounds libraries as well as multiplexed diagnostic and environmental testing and other biochemical assays.
Claims25
A method for encoding and decoding solid supports comprising:
- (a) providing a population of solid supports comprising M batches thereof, wherein M is an integer greater than 1;
- (b) attaching to one or more of said batches one or more fluorophore tag(s), each tag being identifiable by optical interrogation, wherein said one or more tag(s) constitutes a binary code or extended binary code;
- and
- (c) decoding the code composed of the one or more tag(s) on said solid supports by optical interrogation of the tag(s) carried out without isolating the solid supports from each other and without detaching the tags(s) from the solid supports.
The method of claim 13, wherein the ratio of the relative quantities of the fluorophore tags is from about 1:1 to 4:1.
Dependencies are inferred from the claim text.