Method of preparing affinity ligands
AssigneeGE Healthcare Bio-Sciences AB
InventorsJean-Luc Maloisel, Nicolas Thevenin
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Abstract
The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatise the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatised scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.
Claims19
A method of preparing at least one affinity ligand, which method comprises
- (a) providing a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group;
- (b) derivatising the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule;
- and
- (c) opening up the cyclic structure of the derivatised scaffold and adding to the carbonyl carbon an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule;
- to result in a ligand capable of affinity interaction with one site of a target molecule and a second functionality capable of interacting with another site of the same target molecule.
(canceled)
Dependencies are inferred from the claim text.