Compounds and processes for single-pot attachment of a label to nucleic acid
AssigneeMirus Bio Corporation
InventorsPaul Slattum, Jon Wolff, James Hagstrom, Vladimir Budker
Life of this patent
Estimating the term…
Abstract
Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
Claims20
A labeling compound for covalently attaching a label to a nucleic acid in a single-pot reaction comprising:
- an aromatic mustard alkylating group and a label, wherein the compound bears a net neutral charge and wherein the aromatic nitrogen mustard becomes positively charged during the alkylation reaction.
A labeling compound for covalently attaching a label to a nucleic acid in a single-pot reaction comprising:
- an aromatic mustard alkylating group, a label, and a spacer wherein the spacer contains one or more positively charged functional groups that can be reacted with a molecule to convert the functional group to a negatively charged function group after the labeling compound has been covalently attached the nucleic acid.
Dependencies are inferred from the claim text.