Device and method for multiple analyte detection
AssigneeApplera Corporation
InventorsTimothy Woudenberg, Michael Albin, Reid Kowallis, Yefim Raysberg, Robert Ragusa, Emily Winn-Deen
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
Claims8
A method for detecting or quantitating one or more of a plurality of polynucleotide sequences in a liquid sample comprising:
- providing a device comprising a substrate defining a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) a channel in fluid communication with each of the detection chambers and the sample inlet, wherein at least one of the detection chambers contains a first sequence-specific polynucleotide binding polymer for detecting or quantitating a polynucleotide sequence, and at least a different one of the detection chambers contains a second sequence-specific polynucleotide binding polymer for detecting or quantitating a polynucleotide sequence, that differs from the first sequence-specific polynucleotide binding polymer;
- loading a liquid sample into the detection chambers;
- isolating the detection chambers from one another;
- allowing the loaded sample to react with at least one of the sequence-specific polynucleotide binding polymers under conditions effective to produce a detectable signal in at least one of the detection chambers.
Dependencies are inferred from the claim text.