US 2006/0188911 A1Published application

Genetic testing method

AssigneeSysmex Corporation

InventorsYasuhiro Otomo, Kazuki Nakabayashi, Motonari Daito

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Abstract

A genetic testing method for specifically amplifying a DNA complementary to a target nucleic acid existing in a biologic sample, measuring the amplified DNA, and generating diagnosis support information on the basis of a measurement result is described that includes steps of: (a) specifically amplifying a DNA complementary to an internal standard nucleic acid by using a known amount of the internal standard nucleic acid in the absence of the biologic sample component, and obtaining a first measurement result on the basis of the amplification, wherein the DNA has a property to be specifically amplified in the presence of the biologic sample component; (b) specifically amplifying the DNA complementary to the target nucleic acid in the presence of the biologic sample component, and obtaining a second measurement result on the basis of the amplification; and (c) comparing the first and second measurement results to obtain diagnosis support information.

Claims20

Dependent claims

(a) specifically amplifying a DNA complementary to an internal standard nucleic acid by using a known amount of the internal standard nucleic acid in the absence of the biologic sample component, and obtaining a first measurement result on the basis of the amplification, wherein the DNA has a property to be specifically amplified in the presence of the biologic sample component;
(b) specifically amplifying a DNA complementary to the internal standard nucleic acid by using an amount of a nucleic acid having a sequence identical to a sequence of the internal standard nucleic acid in the presence of the biologic sample component, and obtaining a second measurement result on the basis of the amplification, wherein the amount of the nucleic acid is the same as the known amount of the internal standard nucleic acid to be used in the step (a);
(c) specifically amplifying the DNA complementary to the target nucleic acid in the presence of the biologic sample component, and obtaining a third measurement result on the basis of the amplification;
(d) obtaining diagnosis support information on the basis of the third measurement result and a predetermined reference value;
and
(e) determining influence on the diagnosis support information of an amplification inhibitor in the biologic sample component on the basis of the first and second measurement results.

3 more elements

(a) specifically amplifying a DNA complementary to an internal standard nucleic acid by using a known amount of the internal standard nucleic acid in the absence of the biologic sample component, and obtaining a first measurement result on the basis of the amplification, wherein the DNA has a property to be specifically amplified in the presence of the biologic sample component;
(b) specifically amplifying a DNA complementary to the internal standard nucleic acid by using an amount of a nucleic acid having a sequence identical to a sequence of the internal standard nucleic acid in the presence of the biologic sample component, and obtaining a second measurement result on the basis of the amplification, wherein the amount of the nucleic acid is the same as the known amount of the internal standard nucleic acid to be used in the step (a);
(c) specifically amplifying the DNA complementary to the target nucleic acid in the presence of the biologic sample component, and obtaining a third measurement result on the basis of the amplification;
(d) correcting either the third measurement result or a reference value corresponding to a reference target nucleic acid amount on the basis of first and second measurement results;
and
(e) obtaining diagnosis support information on the basis of the corrected one of the third measurement result and the reference value, and the other.

3 more elements

Dependencies are inferred from the claim text.