Primer for nucleic acid detection
AssigneesThe Gov. of the USA as represented by the Secretary of the Dept. of Health and Human, Services, Centers for Disease Control and Prevention
InventorsJothikumar Narayanan, Vincent Hill
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Abstract
This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5′-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable signal from the label when the universal primer hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using the universal primer in nucleic acid amplification, such as real-time PCR.
Claims28
An isolated nucleic acid molecule comprising:
- a 5′ end;
- a 3′ end;
- and
- a label on a nucleotide, wherein the labeled nucleotide is flanked by two nucleotides whose complement nucleotides change a detectable signal from the label when said isolated nucleic acid molecule is hybridized with its complementary nucleic acid molecule.
-5. (canceled)
-16. (canceled)
An isolated nucleic acid molecule comprising 5′-CTCSXXX(n)-3′ (SEQ ID NO:
- 22), wherein a fluorophore is attached to “T” and n comprises 0-11 nucleotides.
-28. (canceled)
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-46. (canceled)
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Dependencies are inferred from the claim text.