Labelled nucleotides
AssigneeSolexa Limited
InventorsColin Barnes, Shankar Balasubramanian, Xiaohai Liu, Harold Swerdlow, John Milton
Life of this patent
Estimating the term…
Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
Claims17
1-8. (canceled)
A method of labeling a nucleic acid molecule, the method comprising incorporating into the nucleic acid molecule a nucleotide or nucleoside molecule, wherein the nucleotide or nucleoside molecule has a base that is linked to a detectable label via a cleavable linker.
A method for determining the sequence of a target single-stranded polynucleotide, comprising monitoring the sequential incorporation of complementary nucleotides, wherein the nucleotides each have a base that is linked to a detectable label via a cleavable linker, and wherein the identity of each nucleotide incorporated is determined by detection of the label linked to the base, and subsequent removal of the label.
A method for determining the sequence of a target single-stranded polynucleotide, comprising,
- (a) providing nucleotides, wherein the nucleotides have a base that is linked to a detectable label via a cleavable linker, and wherein the detectable label linked to each type of nucleotide can be distinguished upon detection from the detectable label used for other types of nucleotides;
- (b) incorporating a nucleotide into the complement of the target single stranded polynucleotide;
- (c) detecting the label of the nucleotide of (b), thereby determining the type of nucleotide incorporated;
- (d) removing the label of the nucleotide of (b);
- and
- (e) optionally repeating steps (b)-(d) one or more times;
- thereby determining the sequence of a target single-stranded polynucleotide.
24-25. (canceled)
Dependencies are inferred from the claim text.