Transposon mediated differential hybridisation
OwnerIan Charles, Duncan Maskellinventors
Life of this patent
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Abstract
A method for identifying an essential gene of an organism comprises: (i) providing a Library of transposon mutants of the said organism; (ii) isolating polynucleotide sequences from the library which flank inserted transposons; (iii) hybridising the said polynucleotide sequences with a polynucleotide library from the said organism; and (iv) identifying a polynucleotide in the said polynucleotide library to which the said polynucleotide sequences do not hybridise, thereby to determine an essential gene of the organism.
Claims27
1-28. (canceled)
A method for identifying an essential gene of an organism comprising:
- (i) providing a library of transposon mutants of the said organism;
- (ii) isolating from the library polynucleotide sequences flanking one side of the inserted transposons to give a first pool of sequences and polynucleotide sequences flanking the other side of the inserted transposons to give a separate second pool of sequences;
- (iii) hybridising the first pool of sequences with a first sample of a polynucleotide library from the said organism and the second pool of sequences with a second sample of the said polynucleotide library from the said organism;
- and
- (iv) identifying a polynucleotide in the said polynucleotide library to which at least one of the said pools of polynucleotide sequences does not hybridise, thereby to determine an essential gene of the organism.
A method for identifying an inhibitor of transcription and/or translation of an essential gene or a conditional essential gene of an organism and/or an inhibitor of activity of a polypeptide encoded by a said gene, which method comprises:
- (a) identifying an essential gene or a conditional essential gene;
- and
- (b) determining whether a test substance can inhibit transcription and/or translation of a gene identified in (a) and/or activity of a polypeptide encoded by a said identified gene, thereby to identify a said inhibitor.
Dependencies are inferred from the claim text.