Method for screening compounds against flaviviruses by using persistent virus-infected cell system
AssigneeBuddhist Tzu Chi General Hospital
InventorLi-Kuang Chen
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Abstract
This invention provides a method for screening compounds against Flavivirus. More specifically, this invention provides a method for screening compounds against Flaviviruses by using a persistent virus-infected cell system, including (a) establishment of persistent virus-infected cell lines, (b) preparation of monoclonal antibody by using said persistent virus-infected cell lines, (c) incubation of tested compounds with said persistent virus-infected cell lines, (d) determination of the inhibition effect of tested compounds or vaccines on Flavivirus by sandwich ELISA using said monoclonal antibody.
Claims25
A method for screening compounds against Flavivirus, comprising the steps of:
- (a) establishing persistent virus-infected cell lines;
- (b) preparing monoclonal antibody by using said persistent virus-infected cell lines;
- (c) incubating tested compounds with said persistent virus-infected cell lines;
- and
- (d) determining an inhibition effect of said tested compounds on said Flavivirus by sandwich ELISA using said monoclonal antibody.
A screening kit for compounds against Flavivirus, comprising:
- (a) materials for establishment of persistent virus-infected cell lines;
- (b) materials for preparation of monoclonal antibody by using said persistent virus-infected cell lines;
- (c) materials for incubation of tested compounds with said persistent virus-infected cell lines;
- and
- (d) materials for determination of an inhibition effect of said tested compounds on said Flavivirus by sandwich ELISA using said monoclonal antibody.
Dependencies are inferred from the claim text.