Enzymatic digestion of tissue
AssigneeAmbion, Inc.
InventorsGary Latham, Brittan Pasloske, Heidi Peltier
Life of this patent
Estimating the term…
Abstract
The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.
Claims59
A method comprising:
- obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid;
- obtaining at least one catabolic enzyme;
- obtaining at least one nuclease inhibitor;
- preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor;
- maintaining the admixture under conditions where the catabolic enzyme is active;
- and
- agitating the admixture;
- wherein the sample is digested to produce a nucleic acid-containing lysate of the sample.
6-10. (canceled)
17-19. (canceled)
22-24. (canceled)
30-39. (canceled)
42-48. (canceled)
(canceled)
53-54. (canceled)
58-59. (canceled)
61-63. (canceled)
A kit for preserving RNA or producing a digested lysate of a tissue sample without homogenizing the sample, comprising, in a suitable container:
- a buffer;
- a catabolic enzyme;
- and
- an ionic detergent.
70-71. (canceled)
73-74. (canceled)
A sample lysis digestion solution comprising:
- at least one cell-containing sample, which comprises a cell-containing nucleic acid;
- at least one a catabolic enzyme;
- at least one nuclease inhibitor;
- and
- a buffer, wherein the buffer includes a pH range of between about 7 and about 10, and wherein the buffer is formulated to maintain the activity of the catabolic enzyme and the nuclease inhibitor,
- wherein the digestion of the cell-containing sample occurs without homogenizing the sample.
(canceled)
82-84. (canceled)
88-89. (canceled)
91-98. (canceled)
Dependencies are inferred from the claim text.