Method of detecting or quantitatively determining mitochondrial dna 3243 variation, and kit therefor

A method for quantifying a DNA having the mitochondrial DNA 3243 mutation, comprising performing quantitative PCR using a DNA obtained from a sample as a template and quantifying an amplification product, wherein the quantitative PCR is a method of using a system in which fluorescence changes depending on amount of an amplification product to quantify the amplification product in PCR on real time basis by measurement of fluorescence and quantifying the DNA in the sample on the basis of the result of the measurement, and primers used for the quantitative PCR comprise a primer having a nucleotide sequence complementary to a nucleotide sequence starting from the nucleotide number 243 in the nucleotide sequence of SEQ ID NO:

• 42 and having a length of 12 to 30 nucleotides.

A nucleic acid probe of comprising an end labeled with a fluorescent dye, wherein a fluorescence of the fluorescent dye decreases upon hybridization, wherein the nucleic acid probe has a nucleotide sequence complementary to a nucleotide sequence starting from nucleotide number 230 in the nucleotide sequence of SEQ ID NO:

• 2 and having a length of 14 to 40 nucleotides, and the 3′ end of the probe is labeled with the fluorescent dye.