Method for detecting an analyte in a sample

A method for detecting an analyte in a sample comprising the steps of

• providing detection oligonucleotides being labeled with a first reporter, which detection oligonucleotides are capable of binding to the analyte,
• providing a solid support,
• providing capture oligonucleotides being bound or capable of binding to the solid support, which capture oligonucleotides are capable of binding to the analyte, thus concentrating the analyte on the solid support,
• contacting the sample with the detection oligonucleotides, the solid support and the capture oligonucleotides, and
• detecting the detection oligonucleotides, wherein
• the detection of detection oligonucleotides is conducted in the presence of quenching oligonucleotides hybridizing to surplus detection oligonucleotides not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection oligonucleotides and/or
• the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a mask obtained from imaging the sample at the emission wavelength of the second reporter and applying this mask to an image of the sample used for detecting the detection oligonucleotides.