Methods and compositions for colorimetrically assessing peptide characteristics
OwnerIleana Nuiry, Roger Micheliinventors
Life of this patent
Estimating the term…
Abstract
Methodologies and compositions useful for calorimetrically assessing peptide characteristics. By way of example, the methodologies can be used to qualitatively and quantitatively assess the degree to which a peptide sample incorporates beta sheet content. The colorimetric information also information is useful to predict solubility, formulating, and other peptide characteristics impacted by beta sheet aggregation. The methodologies can also be practiced for quality control during peptide manufacture. The methodologies can be practiced to determine whether it might be desirable to subject a peptide sample to a deaggregation treatment.
Claims14
A method of processing a peptide, comprising the steps of:
- (a) combining a portion of the peptide with a calorimetric reagent comprising a dye that calorimetrically and selectively interacts more strongly with a first structure of the peptide relative to a second structure of the peptide, wherein the first structure is substantially insoluble in aqueous media at physiological pH, and wherein the second structure is substantially soluble in aqueous media at physiological pH;
- (b) obtaining calorimetric information from step (a) that is indicative of whether the peptide sample contains an undesired amount of the first structure;
- (c) using the information of step (b) to determine whether to subject the peptide to a deaggregation treatment.
Dependencies are inferred from the claim text.