Method and apparatus for UV imaging

A method for analyzing a subject, comprising:

• providing a subject including at least first and second different compounds, said compounds being absorbent of light in a wavelength range of between about 150 nm to about 300 nm;
• illuminating the subject with light in the wavelength range;
• detecting illumination of a first wavelength or range of wavelengths that results from interaction of the light in the wavelength range with the subject so as to capture first 2-dimensional image information of at least a portion of the subject;
• detecting illumination of a second wavelength or range of wavelengths that results from interaction of the light in the wavelength range with the subject so as to capture second 2-dimensional image information of at least a portion of the subject;
• and
• using the first and second 2-dimensional image information to determine concentrations of the first and second compounds in the subject.

A method for analyzing a subject, comprising:

• providing a subject having a compound that fluoresces in response to illumination of a suitable wavelength;
• illuminating the subject with illumination of the suitable wavelength;
• detecting fluorescent illumination emitted by the compound in the subject so as to capture 2-dimensional fluorescent image information;
• and
• determining a quantum yield for the subject based on the 2-dimensional fluorescent image information.

A method for imaging a subject, comprising:

• providing a subject including a living cell and/or a biological material other than artificially crystallized proteins;
• illuminating the subject with illumination having a wavelength of about 300 nm or less;
• and
• detecting autofluorescent illumination emitted by the subject that results from interaction of the illumination with the subject so as to capture 2-dimensional image information of at least a portion of the subject.

A method for imaging a subject, comprising:

• providing a subject;
• illuminating the subject with illumination having a wavelength of about 350 nm or less, the illumination being left circularly polarized;
• detecting illumination that results from interaction of the left circularly polarized illumination with the subject so as to capture first 2-dimensional image information of at least a portion of the subject;
• illuminating the subject with illumination having a wavelength of about 350 nm or less, the illumination being right circularly polarized;
• and
• detecting illumination that results from interaction of the right circularly polarized illumination with the subject so as to capture second 2-dimensional image information of at least a portion of the subject.