Polypeptides for inducing a protective immune response against staphylococcus aureus
OwnerAnnaliesa Anderson, Nelly Kuklin +1inventors
InventorsAnnaliesa Anderson, Nelly Kuklin, Kathrin Jansen
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Abstract
The present invention features hybrid polypeptides providing ORF0657n and ORFO190 epitopes, nucleic acid encoding for the different polypeptides, and a method for evaluating the ability of an immunogen to produce a protective immune response against Staphylococcus infection. ORF0657n and ORF0190 are both S. aureus proteins.
Claims21
A hybrid polypeptide immunogen comprising a modified ORF0657n sequence segment at least about 100 amino acids in length, wherein said modified sequence segment comprises one or more alterations that increases sequence similarity to SEQ ID NO:
- 1.
The hybrid polypeptide of claim 3, wherein said modified amino acid sequence is SEQ ID NO:
- 2 containing 8 to 100 amino acid alterations that increase sequence similarity to SEQ ID NO:
- 1.
A method of making a hybrid polypeptide comprising the step of introducing one or more alterations into a ORF0657n sequence segment at least about 100 amino acids in length, wherein at least one of said alterations increases sequence similarity to SEQ ID NO:
- 1.
A method for evaluating the efficacy of an immunogen to produce a protective immune response against Staphylococcus comprising the steps of:
- (a) inoculating an animal model with said immunogen to produce an immunized animal model;
- (b) challenging said immunized animal model with a Staphylococcus challenge at a potency that provides about 80 to 90% death in said animal model over a period of about 7 to 10 days starting on the first or second day, wherein said Staphylococcus challenge is produced from Staphylococcus grown to stationary phase, and said Staphylococcus challenge is intravenously introduced into said immunized animal model;
- and
- (c) measuring the ability of said immunogen to provide protective immunity.
21-23. (canceled)
Dependencies are inferred from the claim text.