Adenovirus vectors containing cell status-specific response elements and methods of use thereof
OwnerDe Yu, Daniel Hendersoninventors
Life of this patent
Estimating the term…
Abstract
The present invention provides adenoviral vectors comprising cell status-specific transcriptional regulatory elements which confer cell status-specific transcriptional regulation on an adenoviral gene. A “cell status” is generally a reversible physiological and/or environmental state. The invention further provides compositions and host cells comprising the vectors, as well as methods of using the vectors.
Claims38
1-28. (canceled)
A replication-competent adenovirus vector for selective cytolysis of a target cell with a disrupted RB function, comprising:
- an E2F-1 transcriptional regulatory element (TRE) operably linked to an adenovirus gene essential for replication selected from the group consisting of E1A, E1B and E4 and a transgene or a gene that contributes to cytotoxicity or cell death, wherein said vector effects selective cytolysis due to selective replication in a target cell with disrupted RB function.
A replication-competent adenovirus vector for selective cytolysis of a target cell, comprising:
- a cell status-specific transcriptional regulatory element (TRE) selected from a cell cycle-activated TRE or cell-cycle specific TRE, operably linked to an adenovirus gene essential for replication selected from the group consisting of E1A, E1B and E4, wherein said vector effects selective cytolysis of said target cell.
The adenovirus vector of claim 57, wherein said cytokine is GM-CSF.
The adenovirus vector of claim 59, wherein said ADP gene has a sequence presented as SEQ ID NO:5.
Dependencies are inferred from the claim text.